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EFIRM技术被称为基因诊断领域的“狙击枪”,被美国奥巴马政府列为 21 世纪最具挑战的生物技术第三位,并在AAAS2016年会暨全球科学大会上被誉为“癌症登月计划”中的登月火箭。

2009年的《临床癌症研究》、2014年《呼吸与重症监护》以封面文章对EFIRM技术进行了报道。

技术文献列表

1. Wang C, Wang A, Wei F, Wong DTW, Tu M. Electric Field-Induced Disruption and Releasing Viable Content from Extracellular Vesicles. Methods Mol Biol.2017;1660:367-376. doi: 10.1007/978-1-4939-7253-1_30. PubMed PMID: 28828672.

2. Khan RS, Khurshid Z, Yahya Ibrahim Asiri F. Advancing Point-of-Care (PoC) Testing Using Human Saliva as Liquid Biopsy. Diagnostics (Basel). 2017 Jul 4;7(3). pii: E39. doi: 10.3390/diagnostics7030039. Review. PubMed PMID: 28677648;PubMed Central PMCID: PMC5617939.

3. Huang WL, Chen YL, Yang SC, Ho CL, Wei F, Wong DT, Su WC, Lin CC. Liquid biopsy genotyping in lung cancer: ready for clinical utility? Oncotarget. 2017 Mar 14;8(11):18590-18608. doi: 10.18632/oncotarget.14613. Review. PubMed PMID:28099915; PubMed Central PMCID: PMC5392351.

4. Kaczor-Urbanowicz KE, Martin Carreras-Presas C, Aro K, Tu M, Garcia-Godoy F,Wong DT. Saliva diagnostics - Current views and directions. Exp Biol Med(Maywood). 2017 Mar;242(5):459-472. doi: 10.1177/1535370216681550. Epub 2016 Dec 8. Review. PubMed PMID: 27903834; PubMed Central PMCID: PMC5367650.

5. Pu D, Liang H, Wei F, Akin D, Feng Z, Yan Q, Li Y, Zhen Y, Xu L, Dong G, Wan H, Dong J, Qiu X, Qin C, Zhu D, Wang X, Sun T, Zhang W, Li C, Tang X, Qiao Y,Wong DT, Zhou Q. Evaluation of a novel saliva-based epidermal growth factor receptor mutation detection for lung cancer: A pilot study. Thorac Cancer. 2016 Jul;7(4):428-36. doi: 10.1111/1759-7714.12350. Epub 2016 May 13. PubMed PMID:27385985; PubMed Central PMCID: PMC4930962.

6. Tu M, Chia D, Wei F, Wong D. Liquid biopsy for detection of actionable oncogenic mutations in human cancers and electric field induced release and measurement liquid biopsy (eLB). Analyst. 2016 Jan 21;141(2):393-402. doi:10.1039/c5an01863c. Review. PubMed PMID: 26645892; PubMed Central PMCID:PMC4701580.

7. Tang JH, Chia D. Liquid Biopsies in the Screening of Oncogenic Mutations in NSCLC and its Application in Targeted Therapy. Crit Rev Oncog.2015;20(5-6):357-71. doi: 10.1615/CritRevOncog.v20.i5-6.90. PubMed PMID:27279235; PubMed Central PMCID: PMC5544421.

8. Lin CC, Huang WL, Wei F, Su WC, Wong DT. Emerging platforms using liquid biopsy to detect EGFR mutations in lung cancer. Expert Rev Mol Diagn.2015;15(11):1427-40. doi: 10.1586/14737159.2015.1094379. Epub 2015 Sep 30.Review. PubMed PMID: 26420338; PubMed Central PMCID: PMC4889428.

9. Tu M, Wei F, Yang J, Wong D. Detection of exosomal biomarker by electric field-induced release and measurement (EFIRM). J Vis Exp. 2015 Jan 23;(95):52439.doi: 10.3791/52439. PubMed PMID: 25650727; PubMed Central PMCID: PMC4354565.

10. Yang J, Wei F, Schafer C, Wong DT. Detection of tumor cell-specific mRNA and protein in exosome-like microvesicles from blood and saliva. PLoS One. 2014 Nov 14;9(11):e110641. doi: 10.1371/journal.pone.0110641. eCollection 2014. PubMed PMID: 25397880; PubMed Central PMCID: PMC4232306.

11. Wei F, Lin CC, Joon A, Feng Z, Troche G, Lira ME, Chia D, Mao M, Ho CL, Su WC,Wong DT. Noninvasive saliva-based EGFR gene mutation detection in patients with lung cancer. Am J Respir Crit Care Med. 2014 Nov 15;190(10):1117-26. doi:10.1164/rccm.201406-1003OC. PubMed PMID: 25317990; PubMed Central PMCID: PMC5447327.

12. Wei F, Yang J, Wong DT. Detection of exosomal biomarker by electric field-induced release and measurement (EFIRM). Biosens Bioelectron. 2013 Jun15;44:115-21. doi: 10.1016/j.bios.2012.12.046. Epub 2013 Jan 2. PubMed PMID:23402739; PubMed Central PMCID: PMC3809103.

13. Wei F, Liao W, Xu Z, Yang Y, Wong DT, Ho CM. Bio/abiotic interface constructed from nanoscale DNA dendrimer and conducting polymer for ultrasensitive biomolecular diagnosis. Small. 2009 Aug 3;5(15):1784-90. doi:10.1002/smll.200900369. PubMed PMID: 19384878; PubMed Central PMCID: PMC2800851.

14. Wei F, Patel P, Liao W, Chaudhry K, Zhang L, Arellano-Garcia M, Hu S, Elashoff D, Zhou H, Shukla S, Shah F, Ho CM, Wong DT. Electrochemical sensor for multiplex biomarkers detection. Clin Cancer Res. 2009 Jul 1;15(13):4446-52. doi:10.1158/1078-0432.CCR-09-0050. Epub 2009 Jun 9. PubMed PMID: 19509137; PubMed Central PMCID: PMC2799532.

15. Wei F, Wang J, Liao W, Zimmermann BG, Wong DT, Ho CM. Electrochemical detection of low-copy number salivary RNA based on specific signal amplification with a hairpin probe. Nucleic Acids Res. 2008 Jun;36(11):e65. doi:10.1093/nar/gkn299. Epub 2008 May 17. PubMed PMID: 18487624; PubMed Central PMCID: PMC2441804.

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